(a) NADP reductase enzyme is located on .............. .
(b) Breakdown of proton gradient leads to release of ............ .
(a) NADP reductase enzyme is located on the outer side of thylakoid membrane. It is bounded to the thylakoid membrance in light and becomes free in stroma during dark.
(b) ATP Molecules The movement of $\mathrm{H}^{+}$ions across the membrane is coupled with the formation of ATP synthesis in presence of enzyme ATP synthase.
$$\begin{aligned} 3 \mathrm{CO}_2+9 \mathrm{ATP}+6 \mathrm{NADPH}+\text { water } \longrightarrow & \text { Glyceraldehyde } 3-\text { phosphate } \\ + & 9 \mathrm{ADP}+6 \mathrm{NADP}^{+}+8 \mathrm{Pi} . \end{aligned}$$
Analyse the above reaction and answer the following questions
(a) How many molecules of ATP and NADPH are required to fix one molecule of $\mathrm{CO}_2$ ?
(b) Where in the chloroplast does this process occur?
(a) 2 molecules of ATP for phosphorylation and two molecules of NADPH for reduction are required per $\mathrm{CO}_2$ molecule fixed.
(b) The calvin cycle occurs in the stroma of the chloroplast.