Yes, extragenomic DNA is present in both prokaryotes and eukaryotes. In case of eukaryotes, extragenomic DNA is present in two organelles, e.g., plastids and mitochondria.

Extrachromosomal DNA in Prokaryotes In case of prokaryotic cells, the extragenomic DNA is present in the form of plasmids. The plasmid are circular DNA molecules, which confer certain unique phenotypic characters to the bacteria.

One such character is antibiotic resistance to bacteria. The plasmid DNA is also used to monitor bacterial transformation with foreign DNA. Bacteria transformation is the method of sexual reproduction in bacteria.

Extrachromosomal DNA in Eukaryotes Mitochondrial DNA (mtDNA) is usually circular. Mitochondrial DNA spans about 16,500 DNA building blocks (base pairs) representing a small fraction of the total DNA in cells.

mtDNA contains 37 genes, all of which are essential for normal mitochondrial functions. Thirteen of these genes provides instruction for making enzymes involved in oxidative phosporylation.

Mitochondrial genes are among the estimated 20000-25000 total genes in the human genome.

Plastids These are small bodies found free in most plant cells and are of three types leucoplast, chromoplast and chloroplast. These are double membrane bound structures for trapping radiation and storage purpose. It contains small, double stranded circular DNA molecules and ribosomes essential in synthesising certain proteins. As they both can synthesise proteins and replicate on their own they are known as semi-autonomous organelles.

In animals and plants, plasma membrane structure and function are correlated. A cell membrane consists of protein, lipid and carbohydrates.

The protein component present in membrane acts as a solute channels allowing the flow of minerals, hormones and cellular information from one organelle to another or from one cell to another.

In case of plants where minerals are absorbed actively from the soil. The plasma membrane possess proteinaceous carrier. The oligosaccharides attached to membrane serve as recognition centres and help in recognising foreign entities before allowing them entry into the cell.

The lipid content of plasma membrane in plant and animal cells are arranged with their hydrophilic polar head directed outwards and non-polar hydrophobic tails directed inwards providing fluidity to the membrane. Glycocalyx present in cell membrane also helps in cellular attachment.

(a) Cell organelles with no membrane In eukaryotic cell, ribosomes occur freely in the cytoplasmic matrix and are attached to the outer cytoplasmic surface of the rough endoplasmic reticulum and nuclear envelope. The ribosomes are also found in the matrix of mitochondria and stroma of plastids called as mitoribosomes and plastidoribosomes, respectively.

(b) Cell organelles with single membrane A lysosome is a tiny sac bounded by a single unit membrane of lipoprotein. The lysosomes occurs in all animal cells and protozoans. Prokaryotic cell lack lysosomes.

Number of lysosomes varies with the cell type. Lysosome contain a dense, finely granular fluid consisting of glycoproteinaceous hydrolytic (digestive) enzymes called acid hydrolases. Cell organelles like sphaerosomes and microbodies also possess single membranes.

(c) Cell organelles with double membrane Mitochondria are double membrane bound structures with the outer membrane and inner membrane dividing its lumen distinctly into two aqueous compartments.

The inner compartment is called the matrix whereas the outer membrane forms the continuous limiting boundary of the organelle. Chloroplast and nucleus are also double membrane bound organelles.

In bacteria, additional to the genomic DNA, small circular DNA molecules are present in cytoplasm. These small molecules are called plasmids. They confer unique phenotypic character to bacteria, i.e., resistance to antibiotics. It is also used to monitor bacterial transformation with foreign DNA. In eukaryotes, the extra DNA molecules are present in chloroplast (stroma) and mitochondria (matrix). Because of the presence of this DNA molecule, they are treated as self autonomous organelles. In case of highly active organism, the DNA extrachromosomal is found to be more as compared to the less active ones.

Mitochondria is a sausage shaped or cylindrical structure having a diameter of 0.2 to 1.0 $\propto \mathrm{m}$ and length 1.0-4.1 $\propto \mathrm{m}$. Each mitochondrion is a double membrane bound structure with outer membrane and inner membrane dividing its lumen distinctly into two aqueous compartments. The inner compartment is called matrix and outer one forming infoldings is called cristae towards the matrix. These cristae are associated with an increase in surface area.

The mitochondria are the actual sites of aerobic respiration. They produce cellular energy in the form of ATP, hence are called as power house of the cell.

This ATP generated is used in performing vital functions by the organism. The matrix of mitochondria also possess a single circular DNA molecule of itself and a few RNA molecules, ribosomes (70S) and the compartments required for the protein synthesis.