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23
Explain the association of carbohydrate to the plasma membrane and its significance.
Explanation

Sugar residues or carbohydrates attaches either with proteins or lipids usually making up less than $10 \%$ of the membrane weight, they can give rise to a wide variety of structures in relatively short chains. They give individual cell types a distinguishing features. Therefore, they may be involved in.

Cell Recognition e.g., surface of RBC have carbohydrates arranged in branched chains: differences in the arrangement give rise to different blood group antigens (i.e., AB and O ).

Cell surface differences are also responsible for the specificity of action of cells with hormones, drugs, viruses or bacteria. The cause of cell surface differences is related to characteristic surface due to carbohydrate component.

24

Comment on the cartwheel structure of centriole.

Explanation

Centrosome is an organelle usually containing two cylindrical structures called centrioles. They are surrounded by amorphous pericentriolar materials both the centriole in centrosome lie perpendicular to each other in which each has an organisation like that of an 'cartwheel'.

A centriole possess a whorl of 9 peripheral fibrils. There fibrils are absent in the centre, hence the arrangement is called $9+0$. Each fibrils is made of 3 sub-fibres. Therefore, called triplet fibril.

The centrioles form the basal body of cilia and flagella. It also forms spindle fibres that gives rise to spindle apparatus during cell division in animals.

25
Briefly describe the cell theory.
Explanation

Schleiden and Schwann in 1838-39, formulated the cell theory, which originally contained following two statements

(i) All living beings are made up of cells and products formed by the cells.

(ii) Cells are the structural and functional units of life.

The cell theory stated by Schleiden and Schwann failed to explain the question of origin of cells. A major expansion of the cell theory was expressed by Virchow in 1855, in his statement 'Omnis cellula e cellula' (all cells arise from pre-existing cells). This concept, was the actual idea of Nagelli (1846), which later on was elaborated by Virchow, along with considerable evidences in its support. The work of Nagelli and Virchow established cell division as the central phenomenon in the continuity of life.

Thus, the modern cell theory is based on two facts

(i) all living organisms are composed of cells and products of cell.

(ii) cells are the bosic structural and functional unites of life.

(iii) all cells arise from pre-existing cells.

Viruses are exception to cell theory as they are not composed of cell. They consist of a nucleic acid (DNA or RNA) surrounded by a protein sheath and are incapable of independent existence, self regulation and self reproduction.

26
Differentiate between Rough Endoplasmic Reticulum (RER) and Smooth Endoplasmic Reticulum (SER).
Explanation

Difference between Rough Endoplasmic Reticulum (RER) and Smooth Endoplasmic Reticulum (SER) are as below

RER
(Rough Endoplasmic Reticulum)
SER
(Smooth Endoplasmic Reticulum)
1. RER possesses ribosomes attached to its surface. SER does not bear ribosomes attach to their surface.
2. Mainly formed of cisternae and few tubules. Mainly formed of vesicles and the tubules.
3. RER takes part in the synthesis of proteins and enzymes. SER takes part in the synthesis of glycogen, lipids and steroids.
4. RER is internal and connected with nuclear envelope. SER is peripheral. It may be connected with plasmalemma.
5. It may develop from the nuclear envelope. It may develop from RER.
6. Enzymes for detoxification are absent in RER It has the enzymes for detoxification.
7. It forms lysosomes through the agency of Golgi apparatus. SER gives rise to sphaerosomes.

27
Give the biochemical composition of plasma membrane. How are lipid molecules arranged in the membrane?
Explanation

Chemical composition of plasma membrane

Component Composition
Lipids (20-79%)
Proteins (20-70%)
Carbohydrates (1-5%)
Water 20%

Lipids are the major components of the cell membrane as they form the continuous structural frame of the cell membrane. Lipids such as phospholipids, glycolipids, and steroids are found in membranes.

The lipid molecule possess both polar hydrophilic (water loving) and non-polar hydrophobic (water repelling) ends. The hydrophilic region is in the form of a head, while the hydrophobic part contains two tails of fatty acid. Hydrophobic tail is present towards the centre of the membrane. This structures results is the formation of lipid bilayer known as unit membrane/biological membrane/cell membrane.