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4
MCQ (Single Correct Answer)

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

A

DNA can be seen in visible light.

B

DNA can be seen without staining in visible light.

C

Ethidium bromide stained DNA can be seen in visible light.

D

Ethidium bromide stained DNA can be seen under exposure to UV light.

5
MCQ (Single Correct Answer)

'Restriction' in restriction enzyme refers to

A

cleaving of phosphodiester bond in DNA by the enzyme

B

cutting of DNA at specific position only

C

prevention of the multiplication of bacteriophage in bacteria

D

All of the above

6
MCQ (Single Correct Answer)

Which of the following is not required in the preparation of a recombinant DNA molecules?

A

Restriction endonucleases

B

DNA ligase

C

DNA fragments

D

E. coli

7
MCQ (Single Correct Answer)

In agarose gel electrophoresis, DNA molecules are separated on the basis of their

A

charge only

B

size only

C

charge to size ratio

D

All of these

8
MCQ (Single Correct Answer)

The most important feature in a plasmid to be used as a vector is

A

Origin of replication (Ori)

B

presence of a selectable marker

C

presence of sites for restriction endonuclease

D

its size