While isolating DNA from bacteria, which of the following enzymes is not used?
Lysozyme
Ribonuclease
Deoxyribonuclease
Protease
Which of the following has popularised the PCR (Polymerase Chain Reaction)?
Easy availability of DNA template
Availability of synthetic primers
Availability of cheap deoxyribonucleotides
Availability of 'Thermostable' DNA polymerase
An antibiotic resistance gene in a vector usually helps in the selection of
competent cells
transformed cells
recombinant cells
None of these
Significance of heat shock method in bacterial transformation is to facilitate.
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
The role of DNA ligase in the construction of a recombinant DNA molecule is
formation of phosphodiester bond between two DNA fragments
formation of hydrogen bonds between sticky ends of DNA fragments
ligation of all purine and pyrimidine bases
None of the above