Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
All of the above
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
human gene may have intron which bacteria cannot process
amino acid codons for humans and bacteria are different
human protein is formed but degraded by bacteria
All of the above
Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
Which of the following statements does not hold true for restriction enzyme?
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It produces the same kind of sticky ends in different DNA molecules