Significance of heat shock method in bacterial transformation is to facilitate.
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
The role of DNA ligase in the construction of a recombinant DNA molecule is
formation of phosphodiester bond between two DNA fragments
formation of hydrogen bonds between sticky ends of DNA fragments
ligation of all purine and pyrimidine bases
None of the above
Which of the following is not a source of restriction endonuclease?
Haemophilus influenzae
Escherichia coli
Agrobacterium tumefaciens
Bacilius amyloli
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
All of the above
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
human gene may have intron which bacteria cannot process
amino acid codons for humans and bacteria are different
human protein is formed but degraded by bacteria
All of the above