Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
Which of the following statements does not hold true for restriction enzyme?
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It produces the same kind of sticky ends in different DNA molecules
How is copy number of the plasmid vector related to yield of recombinant protein?
The recombinant DNA can multiply as many times as the copy number of the vector plasmid thereby determining the yield of recombinant protein. So, higher the copy number of plasmid vector, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.
Would you choose an exonuclease, while producing a recombinant DNA molecule?
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest and the circular plasmid (vector) will not get cut as it lacks free ends.