The most important feature in a plasmid to be used as a vector is
Origin of replication (Ori)
presence of a selectable marker
presence of sites for restriction endonuclease
its size
While isolating DNA from bacteria, which of the following enzymes is not used?
Lysozyme
Ribonuclease
Deoxyribonuclease
Protease
Which of the following has popularised the PCR (Polymerase Chain Reaction)?
Easy availability of DNA template
Availability of synthetic primers
Availability of cheap deoxyribonucleotides
Availability of 'Thermostable' DNA polymerase
An antibiotic resistance gene in a vector usually helps in the selection of
competent cells
transformed cells
recombinant cells
None of these
Significance of heat shock method in bacterial transformation is to facilitate.
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene