'Restriction' in restriction enzyme refers to
cleaving of phosphodiester bond in DNA by the enzyme
cutting of DNA at specific position only
prevention of the multiplication of bacteriophage in bacteria
All of the above
Which of the following is not required in the preparation of a recombinant DNA molecules?
Restriction endonucleases
DNA ligase
DNA fragments
E. coli
In agarose gel electrophoresis, DNA molecules are separated on the basis of their
charge only
size only
charge to size ratio
All of these
The most important feature in a plasmid to be used as a vector is
Origin of replication (Ori)
presence of a selectable marker
presence of sites for restriction endonuclease
its size
While isolating DNA from bacteria, which of the following enzymes is not used?
Lysozyme
Ribonuclease
Deoxyribonuclease
Protease